![]() The results revealed a potential opportunity for commercial cultivation of edible mushrooms, especially P. eryngii compared to the substrates of cardboard or SCG alone. Combination between cardboard and SCG, especially substrate formulations of 30% cardboard+70% SCG increased mycelial density and number of primordial formations of P. eryngii, while high concentration of yeast extract (5-50 g/l) did not show any beneficial influence. The concentration of 1 g/l of yeast extract showed the best effects on mycelial growth and primordia formation of P. 1, 5, 10, 20, 30 and 50 g/l) as a supplement in potato dextrose agar media (PDA) on P. The efficiency of different yeast extract concentrations (i.e. Studies were carried out using five different substrate formulations viz., 100% cardboard (TS1), 100% SCG (TS2), 50% cardboard+50% SCG (TS3), 70% cardboard+30% SCG (TS4) and 30% cardboard+70% SCG (TS5). Hence, mycelium of king oyster mushroom (Pleurotus eryngii) were experimentally evaluated on cardboard and spent coffee ground (SCG) substrates for the mycelial growth rate, density and primordial formation. An effective tool to identify necessary nutrients for the production of fruiting bodies on the specialty substrates is mycelium growth. – mycelium itself is weak, sieved or home made with a violation of technology.Cultivation of edible mushrooms on urban wastes represents one of the most economically and cost-effective organic recycling processes. Please read how to add mycelium to the substrate here. In the future, they can heat a block, but only by their own development, and growth of oyster mushroom mycelium is suppressed and may cause a death of such block. In places where the mycelium is concentrated there will be local self-heating and active growth, while in other places of the block a competitive microflora will develop – spores of molds or bacteria. In such cases the block tends to overgrow in spots. – Mycelium badly mixed with the substrate or mycelium added in layers (so-called “zebra bags”). After that keep the temperature constant, without swings. Bags after incubation must be moved into a room with a temperature of at least 20 degrees, so that they do not cool down, otherwise the initial growth speed of fungal hyphae decreases significantly. – improper heat treatment of the substrate. This can be caused by the following reasons: If the mushroom block is poorly colonized, and it does not heat, this means that mycelial growth activity of the the oyster mushroom block is very weak. If you have a bizonal cultivation system and you move the blocks from the incubator to the fruiting room, then it is best to do this at the stage of the ” primordial nod or at the beginning of primordia formation. How should primordia look like? Powerful, homogeneous hemispheric formations, appear at the slits and, as a rule, completely fill the perforation area. At first they appear in the form of small dots, the size of poppy seeds, white in color, then the mushroom buds grow to the size of a pinhead and start to become gray. Then mushroom rudiments or primordia appear. It looks like a white fluff or a piece of cotton wool. It begins with the appearance of the so-called primordial nod, which occurs under the film in the immediate vicinity of the perforations. In the photo on the right, the perforations are not open. In a living bag near the perforations, a fluffy layer forms – it is the start of primordia formation. The difference is visible even in the opening of perforations (holes). On the left it is a photo of a fully colonized bag ready for fruiting. It’s hard to explain in words, but in the photos, I think you’ll see the differences – on the right – there is a block overheated during incubation stage, and the white color under the film is faded. With a slight pressure, it is tight to the touch and slightly springy. When mycelium is ready to produce fruit bodies, the substrate becomes uniformly white, the block becomes not loose anymore. At 22ºС, blocks can grow twice as fast as at 18ºС. temperature and other incubation parameters.mycelium quality – the active mycelium grows 4-10 days ahead of weak or sieved one.the composition of raw materials (on husk, overgrowth finishes 2-3 days faster).The term when mycelium fully colonizes the substrate, depends on many factors, in particular: On the 13-18th day after the incubation start, the mycelium completely colonizes the substrate and it is possible to transfer oyster mushroom blocks to initiate fruiting. How to understand that the mushroom block is ready for fruiting?
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